Background and goals
Gelsemium elegans Benth (G. elegans) is a conventional medicinal plant; nonetheless, it’s extremely poisonous, and toxicity varies considerably between species. The reason for this distinction has not been clarified. Humantenirine is a vital poisonous alkaloid in G. elegans, and its metabolism has been poorly studied. This examine aimed to match the completely different metabolites shaped by human liver microsomes, pig liver microsomes, and goat liver microsomes.
Strategies
Excessive-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used to check the metabolism of humantenirine in human liver microsomes, pig liver microsomes, and goat liver microsomes.
Outcomes
A complete of eight metabolites (M1-M8) had been recognized, and three main metabolic pathways had been discovered: demethylation (M1), dehydrogenation (M2, M3, M7), and oxidation (M4, M5, M6, M8).
Conclusions
Based mostly on these outcomes, it’s hypothesized that demethylation is the most important cleansing pathway for humantenirine, offering essential data to raised perceive the metabolism and toxicity variations between species of G. elegans.
Supply:
Journal reference:
Wang, Y., et al. (2024) Comparative Metabolism of the Humantenirine in Liver Microsomes from Pigs, Goats, and People. Future Integrative Medication. doi.org/10.14218/FIM.2024.00029.
